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Further, immunohistochemical studies demonstrate that the triple helical region of collagen V is buried within the fibril, and the amino terminus of α1(V) protrudes from the fibril surface

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After further culturing for 24 h, each well was washed, 1 mg mL −1 MTT solution (100 µL) was added, and the cells were re-incubated for 4 h in the dark

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