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Glutathione S-transferase, C-terminal domain - Wikipedia

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Briefly, RNA substrates were generated by in vitro transcription using the MEGAscript T7 Transcription or MAXIscript SP6/T7 Transcription Kit (Invitrogen) with DNA templates produced through either linearizing plasmids containing the mRNA CDS following an SP6/T7 promoter or annealing chemically synthesized complementary single-stranded DNA oligonucleotides which contain a T7 promoter sequence (Sigma Aldrich Genosys)