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The peptide lysates were desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany)

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In vitro, C16-carnitine has been found to activate specific subsets of neurons in the dorsal root ganglia (DRGs) of muscles that play a key role in sensing muscle fatigue or exertion and are involved in transmitting these sensory signals to the CNS, indicating that C16-carnitine acts as a signaling molecule for muscle afferent nerves [281]