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Relugolix combination therapy maintained symptom relief over 52 weeks, with most patients showing no clinically significant decline in bone mineral density [163]

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For each transfection, 5 µl of Lipofectamine 2000 (11668019, Invitrogen) was added to 100 µl of OptiMEM (Gibco), vortexed briefly and incubated for 5 minutes at room temperature